Zheng, J; Xiao, X; Zheng, S; Liu, J
The purpose of this study is to investigate the effect of four kinds of environmental endocrine disruptors on the proliferation of human umbilical vein endothelial cells (HUVEC) in vitro. HUVEC cells were cultured in estrogen-free improved min. essential medium and divided into several groups based on different treatments: control, 17?-estradiol (E2)group, BPA group, ZEA group, NP group, and DEHP group. Absorbance value (AV) was detd. at 0, 24, 48 and 72 h, and DNA proliferation index (PI) at 72 h. Additive effects of fulvestrant were also obsd. Flow cytometry, terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL) were employed to monitor cell apoptosis. At 24, 48 and 72 h, the AV of E2, BPA, ZEA, NP, DEHP groups were 1.4-1.7 times of controls, resp., and PI were 1.4-1.5 times of controls at 72 h. At the same time, the growth of cells was inhibited by fulvestrant despite the presence of E2, BPA, ZEA, NP, and DEHP. Apoptosis cells were abundant in controls and fulvestrant groups, but little in E2, BPA, ZEA, NP, and DEHP groups. The four environmental estrogens can promote the proliferation of HUVEC cells, which is probably via estrogen receptor pathway or depressed apoptosis.
