US EPA

7484616 

Journal Article 

Anticancer activity of spirulina platensis methanolic extracts against l20b and mcf7 human cancer cell lines 

Fayyad, RJ; Ali, ANM; Dwaish, AS; Al-Abboodi, AKA 

2019 

0 

Plant Archives
ISSN: 0972-5210 

Plant Archives 

19 

1419-1426 

English 

Spirulina platensis was isolated and identified microscopically and genetically through phycocyanin (cpcBA) genes detection and safeguarding the safety of isolated Spirulina platensis through detection of microcystin producing gene (mcyE) gene via PCR technique. To assess the cytotoxic effects of Spirulina platensis hot methanolic extracts on L20B and MCF7 human cancer cell lines, Various concentrations of Spirulina platensis extracts (mg/mL) obtained with 70% methanol solvents were used to treat cell lines after 24 h. and 48 h. exposure time, MTT assay was achieved For cytotoxic effect studies, Chemical analyses and finally GC mass analysis for crude extracts were done to identify the most active chemical compounds, hot methanolic Spirulina extract exhibited notable cytotoxicity against two tested cancer cell lines, the highest percentage (32.5%), (71.5%) of growth inhibition was observed with the treatment using 25 mg/mL, 12.5 mg/ml against L20B and MCF7 respectively. These percentage were increased after 48 hr. application to (35.5%) against L20B, and (78%) against, phytochemical analysis showed that the active chemical compounds from extracts were contains alkaloids, phenols, Terpenes, Steroids, Flavones, Resins, Saponines, proteins, amino acids and tannins. Finally the result of GC mass analysis for extracts proved the existence of many biologically active compounds including 11 anticancer compounds. © 2019 Plant Archives. All rights reserved. 

Anticancer agent; Bioactive compounds; Methanolic extracts; Spirulina platensis