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7495928 
Journal Article 
Cytotoxic influencing activity of latex of Euphorbia antiquoram linn 
Hamsa, D; Sumathi, S; Sowmini, CM; Padma, PR 
2013 
1 
International Journal of Pharmacy and Pharmaceutical Sciences
ISSN: 2656-0097
EISSN: 0975-1491 
5 
2 
130-133 
English 
Cytotoxicity measurements were originally designed for rapid and inexpensive analysis of soluble pharmaceuticals. The brine shrimp lethality assay (BSLA) has been used routinely in the primary screening of the crude extracts as well as isolated compounds to assess the toxicity towards brine shrimp, which could also provide an indication of possible cytotoxic properties of the test materials. Mouse spleen cells have been successfully used to analyze mechanisms of cytotoxicity for a variety of anticancer drugs. The results in BSLA showed that the percentage of lethality rate of the shrimps was found to be less which indicates that at low concentration the survival rate was increased and protected from the effects of test sample. The calculated LC50 value for the tested sample was 20μg. The percentage of viable cells was assessed by MTT assay in a time and dose dependent manner in mouse spleenocytes and LC50 value of methanolic extract of latex milk was found to be 20μg at different time intervals. SRB and Neutral red assay showed that latex milk is safe to normal spleen cells. 
Apoptosis; Cytotoxicity; Euphorbia antiqoram; Medicinal plants