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7500160 
Journal Article 
Carbamylphosphate synthetases in human liver 
Grillo, MA; Pinna, GG; Casolo, L 
1973 
Yes 
International Journal of Biochemistry
ISSN: 0020-711X 
4 
23 
447-450 
English 
1. 1. Human liver contains both a mitochondrial, acetylglutamate-dependent, and a cytosol, acetylglutamate-independent, carbamylphosphate synthetase. 2. 2. The mitochondrial enzyme has Km for NH4+ 23 mM. It is inhibited by DTNB and by bromosuccinimide. 3. 3. The cytosol enzyme is active with NH4+ as well as with glutamine. Its ATP and Mg2+ saturation curve is sigmoidal. UTP inhibits the reaction at low ATP concentrations. © 1973. 
carbamylphosphate synthesis; Human liver; carbamate kinase; in vitro study; liver; theoretical study 
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