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7501905 
Journal Article 
A Thermochemical Study of the Hydrolysis of Urea by Urease 
Jespersen, ND 
1975 
Yes 
Journal of the American Chemical Society
ISSN: 0002-7863
EISSN: 1520-5126 
97 
1662-1667 
English 
Hydrolysis products of the urea-urease system have been deduced from thermochemical measurements. Two distinct sets of products are found which depend upon the buffer used. Classical products (HCO3- and NH4+) are found in phosphate (pH 7.5 and 6.7) and maleate (pH 6.7) buffers. Ammonium carbamate in virtually quantitative yields is obtained in citrate (pH 6.7) and Tris (pH 7.5) buffers. The possible effect of these two sets of products on previous kinetic and thermodynamic studies is discussed in terms of buffer effects. © 1975, American Chemical Society. All rights reserved. 
buffer; urea; urease; article; hydrolysis; kinetics; metabolism; temperature; thermodynamics; Buffers; Hydrolysis; Kinetics; Temperature; Thermodynamics; Urea; Urease