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HERO ID
7501905
Reference Type
Journal Article
Title
A Thermochemical Study of the Hydrolysis of Urea by Urease
Author(s)
Jespersen, ND
Year
1975
Is Peer Reviewed?
Yes
Journal
Journal of the American Chemical Society
ISSN:
0002-7863
EISSN:
1520-5126
Volume
97
Issue
7
Page Numbers
1662-1667
Language
English
DOI
10.1021/ja00840a006
Abstract
Hydrolysis products of the urea-urease system have been deduced from thermochemical measurements. Two distinct sets of products are found which depend upon the buffer used. Classical products (HCO3- and NH4+) are found in phosphate (pH 7.5 and 6.7) and maleate (pH 6.7) buffers. Ammonium carbamate in virtually quantitative yields is obtained in citrate (pH 6.7) and Tris (pH 7.5) buffers. The possible effect of these two sets of products on previous kinetic and thermodynamic studies is discussed in terms of buffer effects. © 1975, American Chemical Society. All rights reserved.
Keywords
buffer; urea; urease; article; hydrolysis; kinetics; metabolism; temperature; thermodynamics; Buffers; Hydrolysis; Kinetics; Temperature; Thermodynamics; Urea; Urease
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