Health & Environmental Research Online (HERO)


Print Feedback Export to File
7540287 
Journal Article 
Water-Soluble Polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of Flora of Krasnoyarsk Krai 
Rovkina, KI; Krivoshchekov, SV; Guryev, AM; Yusubov, MS; Belousov, MV; , 
2018 
1 
Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620
EISSN: 1573-9163 
MAIK NAUKA/INTERPERIODICA/SPRINGER 
NEW YORK 
854-859 
WOS:000459411400005 
Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3-2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa by aqueous extraction (pH = 9) and characterized by physicochemical methods. The content of uronic acids was 13.1 +/- 0.9%, the quantitative content of impurity substances such as protein was 4.25 +/- 0.28%, nucleic acids 0.0056 +/- 0.0003%, and mineral impurities (ash content) 3.45 +/- 0.18%. The PSC was studied by IR spectroscopy. The monomeric composition of PSC was characterized by chromatography-mass spectrometry (major monosugars arabinose, glucose and galacturonic acid). The molecular weight distribution of PSC was studied by the high-pressure exclusion chromatography method. Data demonstrated that the polysaccharide complex of the aerial part of alfalfa consists of three components with molecular weights of 1100 +/- 60, 16 +/- 2 and 7 +/- 1 kDa. The fact that PSC is a mixture of seven polysaccharides was demonstrated by the column ion-exchange chromatography method.