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7548712 
Journal Article 
Asymmetric Bioreduction of Ethyl (S)-4-chloro-3-hydroxy Butanoate Using Dried Baker's Yeast in Aqueous/Ionic Liquid Biphasic System 
Tan, ZQ; Du, H; Han, XX; Yang, ZY; Wu, XD 
2014 
Yes 
Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 
26 
9 
2695-2698 
WOS:000335841000043 
A process of highly stereoselective reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (S)-4-chloro-3-hydroxy butanoate with Baker's yeast was established. Compared with the aqueous/organic solvent biphasic system, the aqueous/ ionic liquid biphasic system could eliminate high toxicity of organic solvent and make the convenience of product separation. Baker's yeast showed the best catalytic activity and enantioselectivity in the aqueous/[Bmim]PF6 biphasic system among the biphasic systems. Under the optimum conditions: [Bmim]PF6 180 g/L, dried baker's yeast 60 g/L, glucose 0.8 mol/L, ethyl 4-chloro-3-oxobutanoate 0.08 mol/L, temperature 30 degrees C, pH 7.0, 24 h and shaking speed 180 rpm, the yield and the e.e. value of (S)-4-chloro-3-hydroxy butanoate reached 92.6 % and 95.4 %, respectively.