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7563212 
Journal Article 
Indolicidin analogs with broad-spectrum antimicrobial activity and low hemolytic activity 
Smirnova, MP; Kolodkin, NI; Kolobov, AA; Afonin, VG; Afonina, IV; Stefanenko, LI; Shpen', VM; Shamova, OV; Kolobov, AA; , 
2020 
1 
Peptides
ISSN: 0196-9781 
ELSEVIER SCIENCE INC 
NEW YORK 
132 
170356 
English 
32593681 
WOS:000569761900003 
To create a broad-spectrum peptide biocide, we synthesized 45 analogs of antimicrobial peptide indolicidin (H-Ile-Leu-Pro-Trp-Lys-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-NH2). Among them the peptides H-Ile-Leu-Pro-(2-Me)Phe-Lys-(2-Me)Phe-Pro-(2-Me)Phe-(2-Me)Phe-Pro-(2-Me)Phe-Arg-Arg-NH2 and HN2-(CH2)10-Ile-Leu-Pro-D-Phe-Lys-D-Phe-Pro-D-Phe-D-Phe-Pro-D-Phe-Arg-Arg-NH2 have the broadest spectrum of antimicrobial activity and the lowest hemolytic activity. They are active against all 11 tested strains of Gram-positive bacteria, Gram-negative bacteria and fungi with MIC50 from 0.9 to 6.1 μg/ml (0.5 to 3.2 μM), being up to 3 times more active than indolicidin, and are at least 1.8 times less hemolytically active than indolicidin (reached the detection limit). These peptides are patented and could be used for further drug development as antimicrobials.