This study was carried out seasonally between December 2017 and September 2018 by selecting five stations from Goksu Stream. In order to determine the water quality of the Goksu Stream, benthic macroinvertebrate fauna, some physicochemical parameters and biotic indices were used. pH, water temperature (°C), DO (dissolved oxygen mg/L), EC (electrical conductivity (μS/cm), salinity (‰) , depth (cm), flow rate (m/s), NH4-N (ammonium nitrogen mg/L), NO2-N (nitrite nitrogen mg/L), NO3-N (nitrate nitrogen mg/L, PO3-P (phosphate mg/L), TP (total phosphorus mg/L), BOD (biochemical oxygen demand mg/L), TSS (total suspended solid mg/L) physicochemical parameters were measured. A number of biotic indices (Biological Monitoring Working Party-BMWP, Average Score Per Taxa-ASPT, Belgian Biotic Index-BBI) and diversity indices (Margalef, Simpson, Shannon Weaver, Simpson Evenness, Shannon Evenness). As a result of this study, a total of 35 taxa were detected. 10 taxa belong to Oligochaeta, 3 taxa belong to Gastropoda, 17 taxa belong to Insecta, 3 taxa belong to Malacos-traca, 1 taxa belong to Polychaeta and 1 taxa belong to Bivalvia. Oligochaeta (61.28 %) was determined to be the most dominant group among benthic ma-croinvertebrates. As a result of the physicochemical variables, macrozoobenthic organisms composition and applied biotic indeces, Goksu Stream has a low quality or high contaminated water quality.