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7612996 
Journal Article 
Determination of Hydrogen Sulfide in Wines Based on Chemical-Derivatization-Triggered Aggregation-Induced Emission by High-Performance Liquid Chromatography with Fluorescence Detection 
Yu, Y; Li, G; Wu, D; Zheng, F; Zhang, X; Liu, J; Hu, N; Wang, H; Wu, Y; , 
2020 
Yes 
Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
EISSN: 1520-5118 
AMER CHEMICAL SOC 
WASHINGTON 
68 
3 
876-883 
English 
31670510 
WOS:000509427900020 
A chemical-derivatization-triggered aggregation-induced emission (AIE) method for the highly selective determination of hydrogen sulfide (H2S) in wine matrices by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed. The detection strategy was developed based on the chemical derivatization of H2S using a low-cost AIE-active fluorescence derivatization reagent, N-(3-iodine-2-oxopropyl)pyrene methamine (NIPM), to trigger specific AIE at 475 nm, which was red-shifted sharply to the maximum emission wavelength as compared with NIPM monomers of 375 nm, effectively quenching the interference from other thiol-containing compounds. With the aid of specific AIE and the effective separation of HPLC, the proposed method showed high selectivity and sensitivity toward H2S. The limits of detection (LODs) at the sub-nM level of 0.25 nmol/L in the wine-beer sample and 0.30 nmol/L in red wine sample were obtained. To certify its applicability, this proposed strategy was successfully applied for the determination of H2S in wine matrices. 
aggregation-induced emission; fluorescence detection; high-performance liquid chromatography; Hydrogen sulfide