Health & Environmental Research Online (HERO)


Print Feedback Export to File
7634905 
Journal Article 
A molecular probe for the detection of homopurine sequences 
Trkulja, I; Biner, SM; Langenegger, SM; Häner, R; , 
2007 
Yes 
ChemBiochem
ISSN: 1439-4227
EISSN: 1439-7633 
WILEY-V C H VERLAG GMBH 
WEINHEIM 
8 
1 
25-27 
English 
17121403 
WOS:000243699600003 
The highly selective detection of homopurine target strands with a triplex-forming molecular probe is described. The binding of a clamp-type oligonucleotide containing two terminally attached pyrene molecules to the target sequence is easily monitored through excimer formation. The oligonucleotide probe allows the efficient discrimination of single nucleotide mismatches because of the high mismatch sensitivity of the triplex formation. © 2007 Wiley-VCH Verlag GmbH & Co KGaA. 
Beacon; DNA; Excimer; Specificity; Triple helix