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7634917 
Journal Article 
Zearalenone sensing with molecularly imprinted polymers and tailored fluorescent probes 
Navarro-Villoslada, F; Urraca, JL; Moreno-Bondi, MC; Orellana, G; , 
2007 
1 
Sensors and Actuators B: Chemical
ISSN: 0925-4005
EISSN: 1873-3077 
ELSEVIER SCIENCE SA 
LAUSANNE 
121 
1 
67-73 
English 
WOS:000244326400008 
A molecularly imprinted polymer (MIP)-based optode has been developed for zearalenone (ZON) mycotoxin analysis. The automated flow-through assay is based on the displacement of tailor-made highly fluorescent tracers by the analyte from a MIP prepared by UV irradiation of a mixture of cyclododecyl 2,4-dihydroxybenzoate (template, ZON mimic), I-allyl piperazine (functional monomer), trimethylolpropane trimethacrylate (crosslinker) and 2,2'-azobisisobutyronitrile in acetonitrile (porogen). Three fluorescent analogues of ZON, namely 2,4-dihydroxybenzoic acid 2-[methyl(7-nitro-benzo[1,2,5]oxadiazol-4-yl)amino]ethyl ester (NBDRA), 2,4-dihydroxy-N-pyren-1-ylmethylbenzamide (PMRA) and of 2,4-dihydroxybenzoic acid 2-[(pyrene-1-carbonyl)amino] ethyl ester (PARA), have been molecularly engineered for the assay development. The pyrene-containing tracers also inform on the characteristics of the microenvironment of the MIP binding sites. PARA has been finally selected to optimize the ZON displacement fluorosensor, that shows a limit of detection of 2.5 x 10(-5) M in acetonitrile. A positive cross-reactivity has been found for beta-zearalenol, but not for resorcinol, resorcylic acid, 17 beta-estradiol, estrone or bisphenol-A. (c) 2006 Elsevier B.V. All rights reserved. 
Fluorescent probes; Fluorosensor; Molecularly imprinted polymers; Pyrene; Zearalenone 
• Resorcinol
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