US EPA

7635487 

Book/Book Chapter 

Implementation, validation and application of a method of evaluation of urinary 1-hydroxypyrene as an indicator of human exposure to polycyclic aromatic hydrocarbons in Rio de Janeiro state, Brazil 

Pires do Rego, EC; Pereira Netto, AD 

2010 

Curran Associates, Inc 

Red Hook, NY 

19th IMEKO World Congress 2009 IMEKO XIX World Congress 

3 

1829-1834 

English 

WOS:000275561600503 

Epidemiological studies have shown that the exposure to polycyclic aromatic hydrocarbons (PAHs) increases the risk of several kinds of human cancers. In this way the use of biomarkers to evaluate PAH exposure is of interest. Urinary 1-hydroxypyrene (1-OHPy) that is the major metabolite of pyrene has been considered as a suitable biomarker of exposure to PAHs as a group, but there are few data about urinary 1-OHPy in Brazil. The objectives of this study were the implementation and validation of a method for the evaluation of urinary 1-OHPy and its application to individuals submitted to environmental exposure to PAHs in Rio de Janeiro State, Brazil. Sixty individuals were studied. The study group was composed of 27 smokers and 33 nonsmokers individuals. The determination of 1-OHPy was performed by high performance liquid chromatography with fluorescence detection. The concentrations of 1-OHPy were corrected by dividing them with the concentrations of urinary creatinine. Row data were statistically evaluated and invalid data or outliers were discarded. The mean concentration of urinary 1-OHPy for smokers (0,387 mu g of 1-OHPy/g of creatinine, n = 25) was more than three times larger than that found for non-smokers (0,116 mu g of 1-OHPy/g of creatinine, n = 27). 

Biomarkers; Environmental; Human exposure; PAH exposure; PAHs; Smoking; Urinary 1-hydroxypyrene 

9781615675937 

19th IMEKO World Congress 

Lisbon, Portugal 

September 6-11, 2009