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7638404 
Journal Article 
Acetic acid/1-ethyl-3-methylimidazolium acetate as a biphasic solvent system for altering the aggregation behavior of collagen molecules 
Yang, H; Li, Q; Liu, S; Li, G; , 
2018 
Yes 
Journal of Molecular Liquids
ISSN: 0167-7322 
ELSEVIER SCIENCE BV 
AMSTERDAM 
262 
78-85 
English 
WOS:000436349300010 
A novel acetic acid/1-ethyl-3-methylimidazolium acetate (AA/[EMIM][Ac]) biphasic solvent system was adopted to alter the aggregation behavior of collagen molecules. Collagen aggregates (0.5 mg/mL) were investigated in the presence of AA/[EMIM][Ac] at various ratios (10:0 to 5:5). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the triple helical structural integrity was maintained integrity across the whole AA/[EMINI][Ac] ratio range. Ultra-sensitive differential scanning calorimetry measurements revealed that the transition temperature varied with the AA/[EMIM][Ac] ratio, indicating a relevant relationship between the aggregated sate of collagen molecules and thermal effects. The surrounding polarity of collagen molecules was examined by fluorescence spectroscopy of pyrene, and disaggregation behavior was enhanced with increasing [EMIM][Ac] ratio, as demonstrated by changes in the size of collagen aggregates determined by dynamic light scattering. Furthermore, the decrease in collagen aggregates was dependent on stronger interactions among collagen molecules. Intrinsic viscosity decreased from 11.526 to 2.607 g/dl, and the Huggins coefficient increased from 0.575 to 4.121 within the AA/[EMIM][Ac] ratio ranging from 10:0 to 5:5. Finally, atomic force microscopy visually confirmed the effect of [EMIM][Ac] on the aggregated state of collagen molecules visually. These findings suggest AA/[EMIM][Ac] could be an efficient solvent system for natural insoluble collagen. (C) 2018 Elsevier B.V. All rights reserved. 
1-ethyl-3-methylimidazolium acetate; Acetic acid; Aggregation behavior; Collagen