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7639334 
Journal Article 
Pyrene biodegradation and its potential pathway involving Roseobacter clade bacteria 
Zhou, H; Zhang, S; Xie, J; Wei, H; Hu, Z; Wang, Hui; , 
2020 
1 
International Biodeterioration & Biodegradation
ISSN: 0964-8305
EISSN: 1879-0208 
ELSEVIER SCI LTD 
OXFORD 
150 
104961 
English 
WOS:000528555000009 
Microbial mineralization plays a significant role in the removal of polycyclic aromatic hydrocarbons (PAHs) from polluted environments. Bacteria affiliated to the Roseobacter Glade are ubiquitous and abundant in various environments, including PAH-polluted areas. However, very lithe is known about the PAH degradation mechanism utilized by the Roseobacter Glade. In this study, eight bacterial strains belonging to the Roseobacter Glade were isolated from sediments collected from the estuary of the Pearl River. Degradation of pyrene and two other typical PAHs (phenanthrene and benzo[a]pyrene) was studied in the eight isolated strains of Roseobacter Glade bacteria (RCB) and three other type strains. The results revealed that all strains had low PAH-degrading efficiency when PAHs were used as the sole source of carbon. However, upon supplementation with an alternative carbon source, the degradation was greatly stimulated. This implies that RCB degrade PAHs via a co-metabolism pathway. A putative pyrene degradation pathway in RCB was re-constructed based on genomic analysis. pahE, a functional marker gene for PAH degradation, was detected in the genomes of RCB. PAH ring-hydroxylating dioxygenase alpha subunit domain family and beta subunit domain family and PAH degradation-related Rieske [2Fe-2S] domain were also identified in the WA strains. These findings provide fundamental evidence that RCB are capable of degrading PAHs through a versatile metabolic pathway. 
Biodegradation; Co-metabolism; Genome; Metabolic pathway; Polycyclic aromatic hydrocarbons