High performance liquid chromatography analysis of D-penicillamine by derivatization with N-(1-pyrenyl)maleimide (NPM) | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

7641662

Reference Type

Journal Article

Title

High performance liquid chromatography analysis of D-penicillamine by derivatization with N-(1-pyrenyl)maleimide (NPM)

Author(s)

Yusof, M; Neal, R; Aykin, N; Ercal, N

Year

2000

Is Peer Reviewed?

Yes

Journal

Biomedical Chromatography
ISSN: 0269-3879
EISSN: 1099-0801

Publisher

WILEY

Location

HOBOKEN

Volume

Issue

Page Numbers

535-540

Language

English

PMID

11113937

DOI

10.1002/1099-0801(200012)14:8<535::AID-BMC11>3.0.CO;2-G

Web of Science Id

WOS:000166001200005

Abstract

D-Penicillamine (2-amino-3-mercapto-3-methylbutanoic acid), a well-known heavy metal chelator, is the drug of choice in the treatment of Wilson's disease and is also effective for the treatment of several disorders including rheumatoid arthritis, primary biliary cirrhosis, scleroderma, fibrotic lung diseases and progressive systemic sclerosis. The method proposed incorporates a technique, previously developed in our laboratory, that utilizes the derivatizing agent N-(1-pyrenyl)maleimide (NPM) and reversed-phase high-performance liquid chromatography (HPLC). The coefficients of variation for within-run precision and between-run precision for 500 nM standard D-penicillamine (D-pen) were 2.27% and 2.23%, respectively. Female Sprague-Dawley rats were given 1 g/kg D-pen i.p. and the amounts of D-pen in liver, kidney, brain and plasma were subsequently analyzed. This assay is rapid, sensitive and reproducible for determining D-pen in biological samples.