Purification of astilbin and isoastilbin in the extract of smilax glabra rhizome by high-speed counter-current chromatography | Health & Environmental Research Online (HERO)

Health & Environmental Research Online (HERO)

Print Feedback Export to File

This record has one attached file:

Citation
Tags

HERO ID

7671469

Reference Type

Journal Article

Title

Purification of astilbin and isoastilbin in the extract of smilax glabra rhizome by high-speed counter-current chromatography

Author(s)

Du, Q; Li, L; Jerz, G

Year

2005

Is Peer Reviewed?

Yes

Journal

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778

Volume

1077

Issue

Page Numbers

98-101

Language

English

PMID

15988992

DOI

10.1016/j.chroma.2005.04.072

Web of Science Id

WOS:000229663400012

Abstract

Purification of astilbin from the rhizome extract of Smilax glabra was conducted using a high-speed counter-current chromatograph equipped with a 700 mL column. In a single operation, 1.5 g of crude sample was separated to yield 105 mg of component astilbin and 48 mg of isoastilbin while the upper phase of the two-phase solvent system composed of n-hexane-n-butanol-water (1:1:2, v/v/v) was used for stationary phase. The chemical structures of the two flavonoid glycosides were confirmed by electrospray ionization ion trap multiple mass spectrometry and nuclear magnetic resonance experiments.

Keywords

Astilbin; Counter-current chromatography; Liliaceae; Purification; Smilax glabra