US EPA

7672838 

Journal Article 

Estrogenic activities of Psoralea corylifolia L. seed extracts and main constituents 

Lim, SH; Ha, TY; Ahn, J; Kim, S 

2011 

1 

Phytomedicine
ISSN: 0944-7113
EISSN: 1618-095X 

18 

5 

425-430 

English 

21382704 

10.1016/j.phymed.2011.02.002 

WOS:000290073100015 

Estrogenic activities of ethanol extract and its active components from Psoralea corylifolia L. were studied using various in vitro assays. The main components from ethanol extract were analyzed to be bakuchiol, psoralen, isobavachalcone, isobavachromene, and bavachinin. In a fractionation procedure, hexane and chloroform fractions showed estrogenic activity in yeast transactivation assay and E-screen assay. In yeast transactivation assay, ethanol extract, hexane, and chloroform fractions showed significantly higher activities at a concentration of 1.0 ng/ml, and bakuchiol at the concentration of 10(-6) M was showed the highest activity, especially, which was higher than genistein at the same concentration. In E-screen assay, cell proliferation of bakuchiol (10(-6) M) showed similar estrogenic activity with genistein (10(-6) M). In ER binding assay, bakuchiol displayed the strongest ER-binding affinity (IC(50) for ERα=1.01×10(-6) M, IC(50) for ERβ=1.20×10(-6) M) and bakuchiol showed five times higher affinity for ERα than for ERβ. 

Bakuchiol; Estrogenic activity; In vitro assays; Psoralea corylifolia L.