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7673102 
Journal Article 
Approach to develop a standardized TLC-DPPH• test for assessing free radical scavenging properties of selected phenolic compounds 
Cieśla, Ł; Kryszeń, J; Stochmal, A; Oleszek, W; Waksmundzka-Hajnos, M 
2012 
Yes 
Journal of Pharmaceutical and Biomedical Analysis
ISSN: 0731-7085
EISSN: 1873-264X 
ELSEVIER SCIENCE BV 
AMSTERDAM 
70 
126-135 
English 
22749343 
WOS:000309314600017 
TLC-DPPH(•) test belongs to a group of frequently performed assays aimed at detection of compounds with desired activity (effect directed analysis). Despite its popularity a standard procedure has not been elaborated so far causing difficulties in comparing results obtained in different laboratories. Thus the aim of the presented research was an approach to develop a standardized procedure for assessing free radical scavenging properties of plant polyphenols. It was observed that specifically positive adsorbent (silica gel) strengthened the observed result of radical-antioxidant reaction, while polar bonded stationary phase CN-silica weakened it. Based on the observed results it was concluded that the TLC-DPPH(•) assay should be preferably performed on the surface of non-specific adsorbents (e.g.: RP-18) with the use of n-hexane for DPPH(•) dissolution. It is also proposed to document the results every 5 min after staining, as they change in time. 
1,1-Diphenyl-2-picrylhydrazyl radical (DPPH); Free radical scavengers; Phenolic compounds; Thin-layer chromatography; TLC-DPPH