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7673309 
Journal Article 
Variability of n-alkanes and nonacosan-10-ol in natural populations of Picea omorika 
Nikolić, B; Tešević, V; Ðorđević, I; Todosijević, M; Jadranin, M; Bojović, S; Marin, PD 
2013 
Yes 
Chemistry & Biodiversity
ISSN: 1612-1872
EISSN: 1612-1880 
10 
3 
473-483 
English 
23495163 
WOS:000316331900015 
This is the first report of population variability of the contents of n-alkanes and nonacosan-10-ol in the needle epicuticular waxes of Serbian spruce (Picea omorika). The hexane extracts of needle samples originated from three natural populations in Serbia (Vranjak, Zmajevački potok, and Mileševka Canyon) were investigated by GC and GC/MS analyses. The amount of nonacosan-10-ol varied individually from 50.05 to 74.42% (65.74% in average), but the differences between the three investigated populations were not statistically confirmed. The results exhibited variability of the composition of n-alkanes in the epicuticular waxes with their size ranging from C(18) to C(35). The most abundant n-alkanes were C(29), C(31), and C(27) (35.22, 13.77, and 12.28% in average, resp.). The carbon preference index of all the n-alkanes (CPI(total)) of the P. omorika populations (average of populations I-III) ranged from 3.3 to 11.5 (mean of 5.9), while the average chain length (ACL) ranged from 26.6 to 29.2. The principal component and cluster analyses of the contents of nine n-alkanes showed the greatest difference for the population growing in the Mileševka Canyon. The obtained results were compared with previous literature data given for other Picea species, and this comparison was briefly discussed. 
Average chain length (ACL); Carbon preference index (CPI); Cluster analysis (CA); Nonacosan-10-ol; Picea omorika; Principal-component analysis (PCA)