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7675843 
Journal Article 
Effect of washing, soaking and pH in combination with ultrasound on enzymatic rancidity, phytic acid, heavy metals and coliforms of rice bran 
Mohammadi, F; Marti, A; Nayebzadeh, K; Hosseini, SM; Tajdar-Oranj, B; Jazaeri, S 
2021 
Yes 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
Elsevier Ltd 
334 
127583 
English 
32711273 
Simultaneous reduction in activity of fat destabilizing enzymes (lipase and lipoxygenase), contaminants heavy metals (As, Cd, Pb, and Hg), antinutrient phytic acid and hazardous coliforms in rice bran was investigated. Application of washing, soaking the washed sample at different pH values (2, 6 and 9) alone or in combination with ultrasonication were examined. While washing was beneficial, its low efficiency acquired further treatment, which was prevailed by application of acidic pH and ultrasound (28 kHz) treatments. Free fatty acids and peroxide value, as indicators of enzymes activity, implied the effectiveness of treatments with adverse impact of sonication on peroxide value. Remarkably, reduction of dominant heavy metals (As, Pb and Zn) and phytic acid were synergistically facilitated by sonication. Coliforms growth was inhibited at pH 2 even at the absence of ultrasonic treatment. Evidently, combination of acidic pH and ultrasound is a practical approach to improve rice bran stability and safety. 
Enzyme activity; Heavy metal; Hydrochloric acid (PubChem CID: 313); Hydrogen peroxide (PubChem CID: 784); Linoleic acid (PubChem CID: 5280450); n-hexan (PubChem CID: 8058); Nitric acid (PubChem CID: 944); pH; Phytic acid; Rice bran; Sodium hydroxide (PubChem CID: 14798); Soybean lipoxygenase (PubChem CID: 135321828); Toluene (PubChem CID: 1140); Triacetin (PubChem CID: 5541); Tween 20 (PubChem CID: 443314); Ultrasound