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HERO ID
7680314
Reference Type
Journal Article
Title
Optimization of extraction method and evaluation of antileishmanial activity of oil and nanoemulsions of Pterodon pubescens benth. fruit extracts
Author(s)
da Silva Santos, É; Garcia, FP; Outuki, PM; Hoscheid, J; Nunes de Goes, PR; Cardozo-Filho, L; Nakamura, CV; Carvalho Cardoso, ML
Year
2016
Is Peer Reviewed?
Yes
Journal
Experimental Parasitology
ISSN:
0014-4894
Publisher
Academic Press Inc.
Volume
170
Page Numbers
252-260
Language
English
PMID
27725158
DOI
10.1016/j.exppara.2016.10.004
Web of Science Id
WOS:000388059400032
Abstract
Currently, leishmaniasis is difficult to manage owing to the limited choice and high toxicity of available drugs, and emergence of drug-resistant protozoa. Medicinal plants, which produce various bioactive molecules, can help counter this global shortage. In this study, we prepared Pterodon pubescens fruit extracts, which show antileishmanial activity, and developed a nanoemulsion of the optimized extract to improve its performance. The extracts were prepared using conventional methods and a supercritical fluid method and were tested for activity against Leishmania amazonensis promastigotes and amastigotes. The two most effective extracts were formulated as nanoemulsions. Although both extracts showed cytotoxicity, the supercritical extracts were more effective against L. amazonensis promastigotes and amastigotes than conventional extracts were. This was attributed to the high content of the geranylgeraniol derivative in the supercritical extracts. The nanoemulsions showed a better selectivity index and significantly improved activity against parasites (IC50: 2.7 μg/mL for nanoemulsion of hexane extract; IC50: 1.9 μg/mL for nanoemulsion of supercritical extract) compared to the Miltefosine standard (0.7 μg/mL). This could be due to the smaller droplets of the supercritical extracts, allowing better penetration. In conclusion, the extracts showed promise in in vitro tests, and could be used as a leishmaniasis treatment.
Keywords
Geranylgeraniol; Leishmania amazonensis; Nanoemulsion; Pterodon pubescens; Supercritical fluid extraction
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