Medicinal attributes of Solanum xanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and anti HIV perspective | Health & Environmental Research Online (HERO)

HERO ID

7680445

Reference Type

Journal Article

Title

Medicinal attributes of Solanum xanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and anti HIV perspective

Author(s)

Kumar, S; Pandey, AK

Year

2014

Is Peer Reviewed?

Yes

Journal

BMC Complementary and Alternative Medicine
ISSN: 1472-6882
EISSN: 14726882

Publisher

BioMed Central Ltd.

Volume

14

Page Numbers

112

Language

English

PMID

24678980

DOI

10.1186/1472-6882-14-112

Web of Science Id

WOS:000334467200002

Abstract

BACKGROUND: Solanum xanthocarpum (Solanaceae) has been used for treatment of many infectious and degenerative diseases in traditional medicine. Present study reports the medicinal efficacy of S. xanthocarpum fruit as antioxidant, anticancer and anti HIV agents.

METHODS: Extracts were prepared using Soxhlet apparatus and partially characterized by thin layer chromatography (TLC). Total flavonoid content was determined spectrophotometrically. Reducing power, DPPH radical scavenging activity and lipid peroxidation inhibition assays were used for measurement of antioxidant potential. Cytotoxic (SRB assay) and anti-HIV RT inhibition (RT assay kit, Roche) activities were determined using ELISA.

RESULTS: TLC revealed the diversity of phytoconstituents in various sequential extracts of S. xanthocarpum fruit. Total flavonoid contents in extracts ranged between 10.22-162.49 μg quercetin equivalent/mg. Spectroscopic scanning of water soluble phenolics showed maximum absorbance at 250 and 280 nm. Polar extracts displayed potent radical scavenging activity (>80%). Several sub-fractions (spots) of extracts separated on TLC plates also exhibited powerful radical scavenging activity. Considerable reducing power was observed in extracts. Hexane fraction provided 55% lipoprotection in rat kidney homogenate. Non-polar extracts exhibited appreciable cytotoxic activity (70-91%) against leukemia (THP-1) and lung cancer (HOP-62) cell lines. Lower inhibitory activity was observed in extracts against HIV Reverse Transcriptase enzyme.

CONCLUSION: The study demonstrated considerable antioxidant and anticancer activities in S. xanthocarpum fruit.

Keywords

Anti HIV; Anticancer; Antioxidant; DPPH; LPOI; Reducing power; Solanum xanthocarpum