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HERO ID
7681347
Reference Type
Journal Article
Title
An antagonistic interaction between A2B adenosine and D2 dopamine receptors modulates the function of rat carotid body chemoreceptor cells
Author(s)
Conde, SV; Gonzalez, C; Batuca, JR; Monteiro, EC; Obeso, A
Year
2008
Is Peer Reviewed?
Yes
Journal
Journal of Neurochemistry
ISSN:
0022-3042
EISSN:
1471-4159
Volume
107
Issue
5
Page Numbers
1369-1381
Language
English
PMID
18823369
DOI
10.1111/j.1471-4159.2008.05704.x
Abstract
We have previously demonstrated that adenosine controls the release of catecholamines (CA) from carotid body (CB) acting on A2B receptors. Here, we have tested the hypothesis that the control is exerted via an interaction between adenosine A2B and dopamine D2 receptors present in chemoreceptor cells. Experiments were performed in vitro in CB from 3 months rats. The effect of A2B adenosine and D2 dopamine agonists and antagonists applied alone or in combination were studied on basal (20%O2) and hypoxia (10%O2)-evoked release of CA and cAMP content of CB. We have found that adenosine A2 agonists and D2 antagonists dose-dependently increased basal and evoked release CA from the CB while A2 antagonists and D2 agonists had an inhibitory action. The existence of A2B-D2 receptor interaction was established because the inhibitory action of A2 antagonists was abolished by D2 antagonists, and the stimulatory action of A2 agonists was abolished by D2 agonists. Further, A2 agonists increased and D2 agonist decreased cAMP content in the CB; their co-application eliminated the response. The present results provide direct pharmacological evidence that an antagonistic interaction between A2B adenosine and D2 dopamine receptors exist in rat CB and would explain the dopamine-adenosine interactions on ventilation previously observed.
Keywords
Adenosine; Adenosine A2B receptors; Carotid body; D2 dopamine receptors; Interactions
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