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7685470 
Journal Article 
BIODEGRADATION OF 2,4,6-TRINITROTOLUENE BY A NOVEL BACTERIUM Serratia nematodiphila-YW: ISOLATION, BIODEGRADING CHARACTERISATION, AND PRODUCTS 
Wu, Y; Yan, Hai; Zhao, Dan; Yin, X; Jia, B; Gao, Y; , 
2013 
1 
Fresenius Environmental Bulletin
ISSN: 1018-4619
EISSN: 1610-2304 
PARLAR SCIENTIFIC PUBLICATIONS (P S P) 
FREISING 
22 
6 
1710-1718 
English 
WOS:000320901100008 
A bacterial strain that can efficiently biodegrade 2,4,6-trinitrotoluene (TNT) was successfully isolated from a contaminated sludge of a sewage pool in a military factory in Hubei Province, China. This strain was identified as Serratia nematodiphila-YW by 16S rDNA sequencing and physiological analysis, which was not previously reported. At an initial concentration of 200 mg/L, 97% of TNT was removed in 4 d in the presence of 1 g/L of glucose, indicating that S. nematodiphila-YW has a strong ability for biodegrading TNT. The gas and liquid chromatography-mass spectrometry results indicated that aminodinitrotoluene isomers, 4-amino-2,6-dinitrotoluene, and 2-amino-4,6-dinitrotoluene were the main intermediate products of the TNT biodegradation by S. nematodiphila-YW, which confirms previous findings. Furthermore another end product, 4-nitro-azo-toluene of C14H10N6O9, was also found in the culture solution, which was a probable product resulting in a distinct pink colour. This pink colour was not previously reported. This study will be important in the basic research and application in the efficient removal of TNT from waste water. 
2, 4, 6-Trinitrotoluene (TNT); Biodegradation; Pink colour; Products; Serratia nematodiphila