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7730203 
Journal Article 
Enzyme aqueous extraction of Moringa oleifera and Canola seed oils and their effect on physiochemical characteristics and stability 
Mehanni, AE; El-Reffaei, WH; Akdowa, EP; Bouba, AA 
2014 
0 
Rivista Italiana delle Sostanze Grasse
ISSN: 0035-6808 
Stazione Sperimentale per le Industrie 
91 
3 
187-197 
English 
WOS:000349815100005 
Aqueous extraction is advantageous over conventional solvent extraction methods because it is neither toxic nor presents any risk of fires and explosion. Viscozyme L, Celluclast 1.5L, Nutrase 0.8L and Alcalase 2.4lFG were screened for their efficacies to extract oil from Moringa oleifera and Canola seeds compared to conventional hexane extraction methods. The hydrolytic enzymes exhibited varying extents of effectiveness for oil extractability. The use of ternary enzyme mixtures enhanced the oil extraction yield but it was still significantly (p<0.05) lower than that obtained by hexane extraction. Cellulases, proteases and pectinases are the most used types of enzymes being the most effective for the aqueous enzymatic oil extraction. Samples of oils treated with (65% Alcalase + 25% Neutrase + 10% Viscozyme) showed that the Rancimat induction period and peroxide values when incubated at 63°C indicated a non significant of oxidative stability compared with those extracted by hexane. 
Moringa oleifera; Canola oil; hexane; enzyme aqueous extraction; oxidative stability