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7731759 
Journal Article 
Liquid chromatographic method for the determination of lutein in milk and pediatric formulas 
Gill, BD; Indyk, HE 
2008 
Yes 
International Dairy Journal
ISSN: 0958-6946 
18 
9 
894-898 
English 
WOS:000258432800002 
A simple and rapid method has been developed for routine compliance testing of lutein in pediatric formulas. Following a mild saponification procedure (70 °C for 10 min) demonstrated to be benign to lutein, lipophilic components were partitioned by a single extraction into hexane:diisopropyl ether (75:25 v/v) and concentrated in mobile phase. Baseline chromatographic separation of lutein and zeaxanthin (internal standard) was achieved using a C30 column and isocratic elution with methanol:dichloromethane (70:30 v/v). Lutein concentration was quantitated against zeaxanthin, which corrected for incomplete extraction recovery. Performance parameters assessed included recovery (101-108%) and repeatability (2.2% RSD). The method was applied to the analysis of lutein-supplemented pediatric formulas, unsupplemented milk powders, and bovine and human milk. © 2008 Elsevier Ltd. All rights reserved.