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7736265 
Journal Article 
Pressurized limonene as an alternative bio-solvent for the extraction of lipids from marine microorganisms 
Golmakani, MT; Mendiola, JA; Rezaei, K; Ibanez, E 
2014 
Yes 
Journal of Supercritical Fluids
ISSN: 0896-8446 
Elsevier 
92 
1-7 
English 
WOS:000341559500001 
A fast and green process for the isolation of high value lipids from different marine microorganisms is presented involving the use of limonene, a green biodegradable solvent, as an alternative to traditional hexane extraction. The optimized process is based on pressurized liquid extraction (PLE) at 200 °C for 15 min using limonene:ethanol (1:1, v/v) as extracting solvent. Under these conditions, lipids were extracted from different microalgae such as Spirulina, Phormidium, Anabaena and Stigeoclonium and their composition in terms of fatty acids were studied by using a Fast-GC-MS method and compared with the original content in the raw material. The extraction method provided the best results in terms of extraction yield for Spirulina, meanwhile the highest amount of ω-3 fatty acids were obtained from Stigeoclonium. © 2014 Elsevier B.V. All rights reserved. 
PLE; Pressurized liquid extraction; Limonene; Spirulina; Anabaena; Stigeoclonium; Phormidium; gamma-Linolenic acid; PUFAs; Compressed fluids