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7737241 
Journal Article 
EVALUATION OF AN HPLC METHOD FOR THE DETERMINATION OF PHYLLOQUINONE (VITAMIN-K-1) IN VARIOUS FOOD MATRICES 
Booth, SL; Davidson, KW; Sadowski, JA 
1994 
Yes 
Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
EISSN: 1520-5118 
42 
2 
295-300 
English 
WOS:A1994MY99000013 
The amount of phylloquinone (vitamin K1) in five different foods was determined by applying a highly sensitive HPLC method that incorporates postcolumn chemical reduction of the quinone followed by fluorescence detection of the hydroquinone form of the vitamin. After initial extraction in 2-propanol and hexane, the food extracts were purified by solid-phase extraction (SPE) on silica gel and analyzed by HPLC or, depending on the matrix, further purified using liquid-phase reductive extraction or reversed-phase SPE on C18. Intra- and interday precision of the assay ranged from 6.6 to 13.6%. The between sample coefficient of variation in the phylloquinone content of 10 random bulk samples ranged from 7.8 to 44.6 %, depending on the food item analyzed. In addition, endogenous dihydrophylloquinone was identified in foods containing hydrogenated oils, although its biological activity is unknown. © 1994, American Chemical Society. All rights reserved.