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7737635 
Journal Article 
Separation of urinary foaming material by bubbling of urine and by gelatinizing of solvent with foam 
Isono, H; Komatsu, M; Suzuki, T; Nojiri, H 
1996 
Eisei Kagaku / Journal of Hygienic Chemistry
ISSN: 0013-273X 
Pharmaceutical Society of Japan 
42 
292-300 
English 
Urine samples are known to foam and gelatinize solvent when shaken. The present work sought to develop a means of separating and characterizing urinary foaming material. A method was developed which can foam and gelatinize solvent. Human urine, after being filtered at pH 10, was bubbled at pH 4-5. The foam was shaken with n-hexane to gelatinize the solvent. The gelatinized solvent layer was washed with water several times, and dried on a glass plate; dried material was then scraped off with a razor. A soft powder was obtained which was suggested to contain protein and sugar. The two moved together electrophoretically at the site of α-globulin, and are suggested to be a complex of protein and sugar (glycoprotein). 
foam separation; solvent gelatinization; urinary foaming material; glycoprotein