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HERO ID
7737635
Reference Type
Journal Article
Title
Separation of urinary foaming material by bubbling of urine and by gelatinizing of solvent with foam
Author(s)
Isono, H; Komatsu, M; Suzuki, T; Nojiri, H
Year
1996
Is Peer Reviewed?
1
Journal
Eisei Kagaku / Journal of Hygienic Chemistry
ISSN:
0013-273X
Publisher
Pharmaceutical Society of Japan
Volume
42
Issue
4
Page Numbers
292-300
Language
English
DOI
10.1248/jhs1956.42.292
Web of Science Id
WOS:A1996VF20400003
Abstract
Urine samples are known to foam and gelatinize solvent when shaken. The present work sought to develop a means of separating and characterizing urinary foaming material. A method was developed which can foam and gelatinize solvent. Human urine, after being filtered at pH 10, was bubbled at pH 4-5. The foam was shaken with n-hexane to gelatinize the solvent. The gelatinized solvent layer was washed with water several times, and dried on a glass plate; dried material was then scraped off with a razor. A soft powder was obtained which was suggested to contain protein and sugar. The two moved together electrophoretically at the site of α-globulin, and are suggested to be a complex of protein and sugar (glycoprotein).
Keywords
foam separation; solvent gelatinization; urinary foaming material; glycoprotein
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