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7738011 
Journal Article 
Determination of phospholipids in vegetable oil by Fourier transform infrared spectroscopy 
Nzai, JM; Proctor, A 
1998 
Yes 
Journal of the American Oil Chemists' Society
ISSN: 0003-021X
EISSN: 1558-9331 
American Oil Chemists' Society 
75 
10 
1281-1289 
English 
WOS:000076782600007 
A method was developed to determine the total phospholipid content in vegetable oil by Fourier transform infrared spectroscopy (FTIR). Calibration curves of 1-α-phosphatidylcholine (PC), 1-α-phosphatidylethanolamine (PE), and 1-α-phosphatidylinositol (PI) in hexane were generated at different concentrations. The optimal phospholipid absorption bands between 1200-970 cm-1 were identified and used for quantitative determination. High R2 ≥ 0.968 were observed between band areas and phospholipid standard concentrations. Phospholipids from crude soybean oil were obtained by water degumming, and purification was performed on a silicic acid column. The phospholipid contents of purified phospholipid extract, degummed and crude soybean oil determined from calibration equations were >90, 0.0113, and 1.77%, respectively. High correlations of determination (R2 ≥ 0.933) were observed between the FTIR method and thin-layer chromatography-imaging densitometry method for the determination of phospholipid content. FTIR was found to be a useful analytical tool for simple and rapid quantitative determination of phospholipids in vegetable oil. 
crude soybean oil (CSBO); Fourier transform infrared (FTIR); phosphatidylcholine (PC); phosphatidylethanolamine (PE); phosphatidylinositol (PI)