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HERO ID
7738795
Reference Type
Journal Article
Title
Novel method to isolate Withaferin A from Withania somnifera roots and its bioactivity
Author(s)
Kannan, ND; Kulandaivelu, G
Year
2007
Is Peer Reviewed?
Yes
Journal
Allelopathy Journal
ISSN:
0971-4693
EISSN:
0974-1240
Publisher
ALLELOPATHY JOURNAL
Location
ROHTAK
Volume
20
Issue
1
Page Numbers
213-220
Language
English
Web of Science Id
WOS:000247449600019
Abstract
Withania somnifera is Ayurvedic medicine to treat depression and inflammation. It contains many phytochemicals (withaferin A, withanine, withanolides, and tropine), however, withaferin A is most active. It was extracted from W. somnifera roots with ethanol and the alcoholic extract was re-extracted in sequence with different solvents (hexane, ether, chloroform, ethyl acetate, and methanol). Each solvent extract was divided into two halves. One kept under room temperature (30±2°C) and the other under low temperature (4±2°C). High yield of crystalline withaferin A was obtained in ether extract at 4±2°C than at room temperature. Withaferin A was tested for antibacterial and antifungal activity at different concentrations. It showed strong activity against Bacillus subtilis at all test concentrations and moderate activity against Escherichia coli and Staphylococcus aureus. It showed similar antifungal activity as ketoconazole against the Aspergillus niger at 200 μg mL-1. This method is inexpensive and appropriate to isolate withaferin A from W. somnifera root. © International Allelopathy Foundation 2007.
Keywords
Aspergillus niger; Bacillus subtilis; biological activity; Candida albicans; Escherichia coli; Pseoudomonas aeruginosa; Rhizopus oryzae; Staphylococcus aureus; withaferin A; Withania somnifera
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