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7743422 
Journal Article 
Spruce Bark as a Source of Antioxidant Active Substances 
Burcova, Z; Kreps, F; Grivnova, P; Strizincova, P; Haz, A; Jablonsky, M; Surina, I; Schmidt, S 
2019 
Yes 
BioResources
ISSN: 1930-2126 
North Carolina State University 
14 
3 
5980-5987 
English 
WOS:000473204700072 
The antioxidant potential of extracts from spruce bark was studied after Soxhlet extraction with ethanol and n-hexane. Ethanol spruce bark extracts were pre-extracted with a mixture of ethanol and n-hexane in a ratio of 1:5. Residues of the extracts and pre-extracts were added to lard (200 mg/kg) to examine its influence on oxidation stability of lard. The composition of the bark extractives was analyzed by GC/MS. The highest antioxidant activity was observed in the original ethanol extracts (15.0 mmol/mg), which had greater antioxidant activity than alpha-tocopherol (13.9 mmol/mg). The n-hexane extract from the spruce bark had 70% less antioxidant activity than the ethanol extract. The high antioxidant activity of the ethanol extract was due to the presence of resin acids (35%) and stilbenes (12%). These antioxidant-active substances increased the oxidation stability of the lard by 5 h, while the n-hexane extract increased the oxidation stability by only 0.5 h. The spruce bark was found to be an alternative feedstock of compounds with potential for use in foodstuffs as antioxidant. © 2019, North Carolina State University. 
Spruce bark; DPPH; Trolox; Antioxidant activity; Induction period; Protection factor