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HERO ID
7745283
Reference Type
Journal Article
Title
A Rapid and reliable thin-layer chromatographic method for the simultaneous estimation of celecoxib and diacerein in their binary mixture using nanosilica gel plate
Author(s)
Rizk, MMS; Toubar, SS; Gadallah, E; Helmy, MIM
Year
2020
Is Peer Reviewed?
Yes
Journal
Journal of Planar Chromatography - Modern TLC
ISSN:
0933-4173
Publisher
Akademiai Kiado ZRt.
Volume
33
Issue
5
Page Numbers
511-522
Language
English
DOI
10.1007/s00764-020-00064-7
Web of Science Id
WOS:000592933200001
Abstract
A novel, rapid, and sensitive stability-indicating high-performance thin-layer chromatography method was developed and validated for the simultaneous determination of celecoxib (CEL) and diacerein (DIA) in bulk and a capsule binary mixture. The complete separation was achieved by the proposed method on nanosilica gel F254 plates using n-hexaneâethyl acetateâtetrahydrofuranâglacial acetic acid (7:3:1:0.8, V/V) as the mobile phase. The chromatographic bands were visualized using short-wave ultraviolet light, and the amount of CEL and DIA was determined by scanning densitometry at 254 nm using the peak area. The RF values were 0.59 and 0.37 for CEL and DIA, respectively. From regression plots, it was found that good linearity was achieved in the concentration ranges of 20.00â320.00 ng/band and 5.00â80.00 ng/band with mean percentage recoveries 99.86 ± 0.70 and 99.64 ± 0.96 for CEL and DIA, respectively. The proposed method was validated according to the International Conference on Harmonisation guidelines. The statistical analysis of the results revealed high accuracy and precision, the suggested procedures could be used as a stability-indicating assay for the simultaneous determination of CEL and DIA in bulk drug and their capsule binary mixture in the presence of their degradation products. © 2020, Akadémiai Kiadó, Budapest, Hungary.
Keywords
Celecoxib; Diacerein; Stability-indicating; HPTLC; Nanoplate
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