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HERO ID
7745507
Reference Type
Journal Article
Title
HPTLC-densitometric analysis of selected antidiabetic drugs in presence of their degradation products
Author(s)
Ahmed, HM; Omar, MA; Batakoushy, HA; Hamid, MAA
Year
2020
Is Peer Reviewed?
1
Journal
Microchemical Journal
ISSN:
0026-265X
EISSN:
1095-9149
Publisher
Elsevier Inc.
Volume
154
Language
English
DOI
10.1016/j.microc.2019.104560
Web of Science Id
WOS:000527366200032
Abstract
In recent years, a lot of single-pill combinations (SPC) manufactured and are used as a promising choice in diabetes treatment. However, this trend made a serious challenge to drug analysts because of the difficulty in the analysis of two or more drugs in the presence of each other. In this study, a new validated high performance thin-layer chromatography was developed for simultaneous determination of dapagliflozin and saxagliptin in their pure form and dosage form and also successively applied as stability indicating assay of both drugs. The proposed method was based on coupling high-performance thin-layer chromatography with dual wavelength detection at 225 and 210 nm for dapagliflozin and saxagliptin, respectively. Chromatographic separations were performed on silica gel 60-F254 aluminum plates with a mobile phase of hexane/methanol/ethyl acetate (4:2:4, v/v/v). The retention factor values were 0.6, 0.18 and a correlation coefficient of 0.9994 and 0.9995 for dapagliflozin and saxagliptin, respectively with a linear range of 50â550 ng/spot for both drugs. The optimum chromatographic conditions were applied to commercial tablet with good percent recovery values, (99.84±1.43) and (99.43±0.63) for dapagliflozin and saxagliptin, respectively. The suggested method could be applied for the studied drugs in quality control-lab as well as in their pharmacokinetic studies. © 2019 Elsevier B.V.
Keywords
Dapagliflozin; Saxagliptin; Stability indicating; Separation; Dosage form
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