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7750774 
Journal Article 
SIMULTANEOUS DETERMINATION OF BUZEPIDE, PHENYLPROPANOLAMINE, AND CLOCINIZINE IN PHARMACEUTICAL PREPARATIONS BY ION-PAIR REVERSED-PHASE HPLC 
Cavazzutti, G; Gagliardi, L; Deorsi, D; Tonelli, D 
1995 
Yes 
Journal of Liquid Chromatography
ISSN: 0148-3919 
18 
2 
227-234 
English 
WOS:A1995QE32500002 
A rapid, accurate and precise method for the determination of buzepide, phenylpropanolamine, and clocinizine in a cough-mixture is described. The method is based on reversed-phase ion-pair HPLC. The only sample preparation necessary for the analysis is its dilution with the mobile phase. The resulting solution is analyzed on a column packed with Lichrosorb RP-18 (10μm) with acetonitrile in aqueous 5 10-2M sodium perchlorate, pH 3.0 (15:85) as initial mobile phase (2 mL·min-1) and detection at 220 and 254 nm. Then a linear gradient up to 95% acetonitrile in 20 min, is applied. The detection limits are 500 ng injected for phenylpropanolamine, 40 and 5 ng for buzepide and clocinizine, respectively. Copyright © 1995 by Marcel Dekker, Inc.