US EPA

7752152 

Journal Article 

Simultaneous determination of five phosphates in dairy products by ion chromatography 

Xie, H; Guo, Lan; Yan, A; Liu, Fan; Wan, Y 

2020 

Yes 

Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 

Bellwether Publishing, Ltd. 

43 

17-18 

770-776 

English 

10.1080/10826076.2020.1811726 

WOS:000567952000001 

A simple and reliable analytical method was established for the quantitative determination of five phosphates (orthophosphate, pyrophosphate, trimetaphosphate, tripolyphosphate, and hexametaphosphate) in dairy products by ion chromatography (IC). The samples were deproteinized with 2% trifluoroacetic acid, defatted with n-hexane, and then purified on a C18 solid phase extraction cartridge. Five phosphates were separated on an IonPacTM AS11-HC analytical column (250 mm × 2 mm) and an IonPacTM AG11-HC guard column (50 mm × 2 mm) with gradient elution of 30-60 mmol/L NaOH, then detected by a suppressor type conductivity detector. The peak areas were linearly valuated with the concentration of the five compounds with good linear coefficients (not less than 0.9986). The limits of detection ranged from 0.1 mg/L to 0.6 mg/L. The average recoveries were within the range of 83.0–117.8% with the RSDs below 9.0%. The concentration of the five phosphates in the treated samples remained relatively stable within 48 h. The proposed procedure has been applied to the determination of the five analytes in several dairy product samples with satisfactory results. © 2020 Taylor & Francis Group, LLC. 

Dairy products; ion chromatography; phosphates