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7753548 
Journal Article 
DEVELOPMENT AND VALIDATION OF A NEW HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR ENANTIOSEPARATION OF DORZOLAMIDE HYDROCHLORIDE ON A COATED CELLULOSE PHENYLCARBAMATE CHIRAL STATIONARY PHASE 
Shamsipur, M; Abdollahpour, A; Heydari, R 
2011 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
TAYLOR & FRANCIS INC 
PHILADELPHIA 
34 
14 
1367-1380 
English 
WOS:000296230900006 
A simple, accurate, precise, and sensitive method using coated cellulose phenylcarbamate chiral stationary phase for separation of dorzolamide hydrochloride (4S,6S) and its enantiomer (4R,6R) in commercial drops has been established. The mobile phase consisted of a mixture of n-hexane and 2-propanol (50:50v/v) containing 0.1% diethylamine and used a flow rate of 1mL min -1. The influence of temperature, flow rate, type, and concentration of organic components and diethylamine in the mobile phase on the retention and enantioselectivity was investigated. The method was validated for linearity, repeatability, accuracy, and limits of detection (LOD) and quantification (LOQ). The calibration range of quantitation for dorzolamide (4S,6S) and its enantiomer (4R,6R) was 0.5-10μgmL -1 and 0.2-5μgmL -1, respectively. The LOD and LOQ were found to be 0.2 and 0.5μgmL -1 for dorzolamide hydrochloride (4S,6S) and 0.1 and 0.2μgmL -1 for its enantiomer (4R,6R), respectively. Repeatability (n=6) showed less than 0.5% relative standard deviation. Copyright © Taylor & Francis Group, LLC. 
cellulose phenylcarbamate chiral stationary phase; dorzolamide hydrochloride; enantiomers; enantioseparation; high performance liquid chromatography; validation