US EPA

7754500 

Journal Article 

Asymmetric synthesis of (-)-1-trimethylsilyl-ethanol with immobilized Saccharomyces cerevisiae cells in water/organic solvent biphasic system 

Lou, WY; Zong, MH; Fan, XD 

2003 

Yes 

Chinese Journal of Chemical Engineering
ISSN: 1004-9541 

CHEMICAL INDUSTRY PRESS 

BEIJING 

11 

2 

136-140 

English 

WOS:000182642800003 

Abstract Asymmetric synthesis of (-)-1-triniethylsilyl-ethanol with immobilized Saccharomyces cerevisiae cells in water/organic solvent biphasic system was studied. The effects of shake speed, hydrophobicity of organic solvent, volume ratio of water phase to organic phase, pH value of aqueous phase and reaction temperature on the initial reaction rate, maximum yield and enantiomeric excess (ee) of the product were systematically explored. All the above-mentioned factors had significant influence on the reaction. n-Hexane was found to be the best organic solvent for the reaction. The optimum shake speed, volume ratio of water phase to organic phase, pH value and reaction temperature were 150 r·min-1, 1/2, 8 and 30°C respectively, under which the maximum yield and enantiomeric excess of the product were as high as 96.8% and 95.7%, which are 15% and 16% higher than those of the corresponding reaction performed in aqueous phase. To our best knowledge, this is the most satisfactory result obtained. 

(-)-1-trimethylsilyl-ethanol; immobilized cell; Saccharomyces cerevisiae; asymmetric synthesis; water/organic solvent biphase