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7755766 
Journal Article 
Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae 
Hajimahmoodi, M; Faramarzi, M; Mohammadi, N; Soltani, N; Oveisi, MR; Nafissi-Varcheh, N 
2010 
Yes 
Journal of Applied Phycology
ISSN: 0921-8971
EISSN: 1573-5176 
SPRINGER 
DORDRECHT 
22 
1 
43-50 
English 
WOS:000274435300005 
Antioxidant activities of both cells and extracellular substances were evaluated in 12 soil-isolated strains of microalgae according to FRAP and DPPH-HPLC assays. Their total phenolic contents were also determined by Folin-Ciocalteu method. Extractions were performed with hexane, ethyl acetate, and water. The results of FRAP assay showed that algal cells contained considerable amounts of antioxidants from 0.56 ± 0.06 to 31.06 ± 4.00 μmol Trolox g-1 for Microchaete tenera hexane extract and Chlorella vulgaris water extract, respectively. In water fractions of extracellular substances, the antioxidants were from 1.30 ± 0.15 μmol Trolox g-1 for Fischerella musicola to 73.20 ± 0.16 μmol Trolox g-1 for Fischerella ambigua. Also, DPPH-HPLC assay represented high antioxidant potential of water fractions. The measured radical-scavenging activities of the studied microalgae were at least 0.15 ± 0.02 in Nostoc ellipsosporum cell mass to a maximum of 109.02 ± 8.25 in C. vulgaris extracellular substance. The amount of total phenolic contents varied in different strains of microalgae and ranged from zero in hexane extract to 19.15 ± 0.04 mg GAE g-1 in C. vulgaris extracellular water fraction. Significant correlation coefficients between two measured parameters indicated that phenolic compounds were a major contributor to the microalgal antioxidant capacities. © Springer Science+Business Media B.V. 2009. 
Antioxidant property; Folin-Ciocalteu method; FRAP assay; DPPH-HPLC assay; Microalgae; Phenolic content