Health & Environmental Research Online (HERO)


Print Feedback Export to File
7756262 
Journal Article 
Extraction, Purification and Antiradical Activities of Alpinetin and Cardamomin from Alpinia katsumadai Hayata 
Lu, HT; Zou, YL; Deng, R; Shan, H 
2013 
Yes 
Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 
ASIAN JOURNAL OF CHEMISTRY 
SAHIBABAD 
25 
17 
9503-9507 
English 
WOS:000330238700017 
Alpinetin and cardamomin are the major effective ingredients, which exist in Alpinia katsumadai Hayata and have strong activity in inhibiting platelet aggregation and tumor formation, antiinflammatory and antibacterial activities. Using orthogonal experiments L16 (43), alpinetin and cardamomin were extracted from Alpinia katsumadai Hayata by microwave-assisted extraction. Then, high-speed countercurrent chromatography (HSCCC) was used to separate and isolate of alpinetin and cardamomin by two-phase solvent system of n-hexane-ethyl acetate-ethanol-water (5:6:5:5, v/v) in one-step separation. The lower phase was used as the mobile phase in the head-totail elution mode. From 158.4 mg of the crude extract, 14.47 mg of alpinetin and 1.72 mg of cardamomin with purities of 99.01 and 97.71 % were obtained, respectively. They were identified by melting points, UV, IR, 1H and 13C NMR. Furthermore, they were tested for 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radicals scavenging activities. The results showed that alpinetin had strong DPPH free radicals scavenging activities. 
Alpinia; Cardamomin; Alpinia katsumadai Hayata; Purification; DPPH free radicals scavenging activities