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HERO ID
7760723
Reference Type
Journal Article
Title
Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding
Author(s)
Li, H; Li, S; Abdelkhalik, S; Shahzad, A; Gu, J; Yang, Z; Ding, M; Liu, K; Zhao, H; Yang, M
Year
2020
Is Peer Reviewed?
Yes
Journal
BMC Plant Biology
ISSN:
1471-2229
Volume
20
Issue
1
Page Numbers
246-246
Language
English
PMID
32487057
DOI
10.1186/s12870-020-02458-5
Abstract
BACKGROUND: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past 20 years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines. RESULTS: F1s and selected F2 and F3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (5060 cm). These DH lines were developed within 4 years through at least 1 year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or 3 years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F2 > F3 > F1. CONCLUSIONS: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.
Keywords
doubled haploids; haploidy; heterosis; hybrids; male sterility; males; outcrossing; plant biology; plant height; powdery mildew; stripe rust; wheat; China
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