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Citation
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HERO ID
7782659
Reference Type
Journal Article
Title
Modification of the immobilized metal affinity electrophoresis using sodium dodecyl sulfate polyacrylamide gel electrophoresis
Author(s)
Lee, BS; Jayathilaka, GD; Huang, JS; Decresce, D; Borgia, JA; Zhou, X; Gupta, S
Year
2008
Is Peer Reviewed?
Yes
Journal
Electrophoresis
ISSN:
0173-0835
EISSN:
1522-2683
Volume
29
Issue
15
Page Numbers
3160-3163
Language
English
PMID
18633940
DOI
10.1002/elps.200800024
Abstract
Modification to the original immobilized metal affinity electrophoresis (IMAEP) technique is presented. SDS-PAGE is used instead of native PAGE for improved extraction of phosphoproteins from a mixture of proteins. Protein samples treated with 2% w/v SDS instead of native sample buffer ensure that proteins are negatively charged. These negative charges on the proteins assure that the proteins migrate electrophoretically towards the anode regardless of their pI values and hence pass through the region embedded with the metal ions. Another benefit of treating proteins with SDS is that it unfolds the phosphoproteins exposing the phosphate groups to facilitate the metal-phosphate interactions. Phosphorylated ovalbumin can only be extracted after SDS sample buffer treatment. Data show that there is no detrimental effect upon SDS treatment on the extraction of phosphoproteins from a mixture of proteins. Electrophoretic migration of phosphoproteins ceases upon encounter with metal ions like Al+3, Ti+3, Fe+3, Fe+2, and Mn+2 whereas non-phosphorylated proteins migrate freely.
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