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7817970 
Journal Article 
Supermolecular structure of photosystem II and location of the PsbS protein 
Nield, J; Funk, C; Barber, J 
2000 
1 
Philosophical Transactions of the Royal Society of London. B: Biological Sciences
ISSN: 0962-8436
EISSN: 1471-2970 
355 
1402 
1337-1344 
English 
11127988 
WOS:000165153100002 
This paper addresses the question of whether the PsbS protein of photosystem two (PS II) is located within the LHC II PS II supercomplex for which a three-dimensional structure has been obtained by cryoelectron microscopy and single particle analysis. The PsbS protein has recently been implicated as the site for non-photochemical quenching. Based both on immunoblotting analyses and structural considerations of an improved model of the spinach LHC II PS II supercomplex, we conclude that the PsbS protein is not located within the supercomplex. Analyses of other fractions resulting from the solubilization of PS Il-enriched membranes derived from spinach suggest that the PsbS protein is located in the LHC II-rich regions that interconnect the supercomplex within the membrane.