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7856534 
Journal Article 
1-Fluoro-2:4-dinitrobenzene as a cytochemical reagent. I. Histological applications 
Maddy, AH 
1961 
Yes 
Experimental Cell Research
ISSN: 0014-4827
EISSN: 1090-2422 
22 
C 
169-180 
English 
13765052 
1. 1. A method for the application of FDNB as a cytochemical reagent is described. The tissue sections are reacted with FDNB in aqueous ethanol containing sodium bicarbonate and the nitro groups of the treated sections converted into a suitably coloured derivative by reduction, diazotisation and coupling with a phenol. 2. 2. From a survey of a wide range of tissue sections it was observed that when used alone, FDNB produces a dense overall reaction, but when it is preceded by the blocking agent benzoyl chloride, the reaction is confined to cell nuclei and a few characteristic cytoplasmic sites. The conditions under which this reaction after blocking takes place and the mechanism of the reaction are discussed. © 1961. 
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