HPLC determination of neurotoxin beta-N-oxalyl-L-alpha,beta-diaminopropionic acid and its alpha-isomer in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

7857406

Reference Type

Journal Article

Title

HPLC determination of neurotoxin beta-N-oxalyl-L-alpha,beta-diaminopropionic acid and its alpha-isomer in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene

Author(s)

Wang, F; Chen, X; Chen, Q; Qin, XC; Li, ZX

Year

2000

Is Peer Reviewed?

Journal

Chinese Chemical Letters
ISSN: 1001-8417
EISSN: 1878-5964

Volume

Issue

Page Numbers

435-438

Language

English

Web of Science Id

WOS:000087193800022

Abstract

A rapid and simple method is presented for determining β-N-oxalyl- α , β -diaminopropionic acid (β-ODAP) and its much less toxic α -isomer (α -ODAP) in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid Chromatographic method for the separation of the derivatives in the pmol range is reported.

Keywords

HPLC; Lathyrus sativus; 1-fluoro-2,4-dinitrobenzene; alpha- and beta-N-oxalyl-alpha,beta-diamino propionic acid; neurotoxin