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HERO ID
7860143
Reference Type
Journal Article
Title
SPECTROPHOTOMETRIC ASSAY USING ORTHO-PHTHALDIALDEHYDE FOR DETERMINATION OF REACTIVE LYSINE IN DAIRY-PRODUCTS
Author(s)
Vigo, MS; Malec, LS; Gomez, RG; Llosa, RA
Year
1992
Is Peer Reviewed?
Yes
Journal
Food Chemistry
ISSN:
0308-8146
EISSN:
1873-7072
Publisher
ELSEVIER SCI LTD
Location
OXFORD
Volume
44
Issue
5
Page Numbers
363-365
Language
English
DOI
10.1016/0308-8146(92)90269-8
Web of Science Id
WOS:A1992HY55400008
Abstract
The applicability of a simple and rapid spectrophotometric assay using o-phthaldialdehyde (OPA) to determine reactive lysine in dairy products was evaluated. For this purpose the values of reactive lysine of model systems and dairy products obtained by this procedure were compared with those obtained by the 1-fluoro-2,4-dinitrobenzene (FDNB) method in dialyzed and non-dialyzed samples. As a reference the latter method was applied to dialyzed samples to avoid interference of sugars. The results obtained showed that the OPA procedure is reliable for use even in the presence of high levels of sugars and can also be used to evaluate lysine damage after Maillard-type reactions. © 1992.
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