Health & Environmental Research Online (HERO)


Print Feedback Export to File
7918048 
Journal Article 
Rapid Method for Determination of beta-Carotene in Foods Using Ultra High Performance Liquid Chromatography 
Ha, J; Shim, Y; Seo, H; Nam, H; Ito, M; Nakagawa, H 
2010 
1 
Food Science and Biotechnology
ISSN: 1226-7708
EISSN: 2092-6456 
19 
5 
1199-1204 
English 
WOS:000283904600010 
The ultra high performance liquid chromatography (u-HPLC) method for determination of β-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column C18 (particle size 2 μm, i.d. 2 mm, length 50 mm), followed by ultra violet (UV) detection at 450 nm. The recovery of β-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 μg/mL for β-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 μg/mL for β-carotene without BHT, respectively. The calibration graph for β-carotene was linear from 0.1 to 25.0 μg/mL for u- HPLC. The intra- and interday precisions (relative standard deviations) were <7.5 and <7.8%, respectively. Benefits of u-HPLC analysis of β-carotene in foods is reduction of the analysis time to approximately 1/4, saving the volume of solvent to approximately 1/15. It seems that u-HPLC can offer significant improvements in speed, sensitivity, and resolution compared with conventional HPLC, this bodes well for future applications. © KoSFoST and Springer 2010. 
beta-carotene; rapid determination; ultra high performance liquid chromatography