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7946884 
Journal Article 
Microbial degradation of petroleum hydrocarbons in a polluted tropical stream 
Adebusoye, SA; Ilori, MO; Amund, OO; Teniola, OD; Olatope, SO 
2007 
1 
World Journal of Microbiology and Biotechnology
ISSN: 0959-3993
EISSN: 1573-0972 
23 
8 
1149-1159 
English 
WOS:000248330300014 
Crude oil degradation was observed in water samples from three sites along the course of a polluted stream in Lagos, Nigeria. Consistent increase and decrease in the total viable counts (TVCs) of indigenous organisms occurred in the test and control experiments, respectively. Enrichments of the water samples with crude oil resulted in the isolation of nine bacteria belonging to seven genera. A mixed culture was developed from the assemblage of the nine species. The defined microbial consortium utilized a wide range of pure HCs including cycloalkane and aromatic HCs. Utilization of crude oil and petroleum cuts, i.e., kerosene and diesel resulted in an increase in TVC (till day 10) concomitant with decreases in pH and residual oil concentration. Crude oil, diesel and kerosene were degraded by 88, 85 and 78%, respectively, in 14 days. Substrate uptake studies with axenic cultures showed that growth was not sustainable on either cyclohexane or aromatics while degradation of the petroleum fractions fell below 67% in spite of extended incubation period (20 day). From the GC analysis of recovered oil, while reductions in peaks of n-alkane fractions and in biomarkers namely n-C17/pristane and n-C18/phytane ratios were observed in culture fluids of pure strains, complete removal of all the HC components of kerosene, diesel and crude oil including the isoprenoids was obtained with the consortium within 14 days. 2007 Springer Science+Business Media B.V. 
bacteria; degradation; hydrocarbons; phytane; pristane; residual oil