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7950069 
Journal Article 
The conversion of fibrinogen to fibrin. VI. Flow birefringence studies on clotting systems inhibited by hexamethylene glycol 
Foster, JF; Samsa, EG; Shulman, S; Ferry, JD 
1951 
Yes 
Archives of Biochemistry and Biophysics
ISSN: 0003-9861
EISSN: 1096-0384 
34 
2 
417-423 
English 
14904077 
1. 1. Flow birefringence measurements have been made on inhibited clotting systems prepared by allowing bovine fibrinogen and thrombin to react in the presence of 0.5 M hexamethylene glycol and diluting the stock solution, which contains an intermediate polymer previously identified, to give various dilutions in 20 and 40% glycerol at constant glycol, salt, and buffer. 2. 2. The dependence of extinction angle on velocity gradient shows the presence of elongated particles of different lengths in the neighborhood of 4000 A., and the moderate dependence of extinction angle on concentration indicates that these particles do not disaggregate when solutions containing glycerol are diluted. 3. 3. The dependence of phase difference on concentration and velocity gradient also indicates that the particles do not change in length with dilution, at least down to a protein concentration of 1 g./l. 1951.